Active Recombinant Mouse Gsk3b protein, His-tagged

• Cat.No.: Gsk3b-2521M
• Product Overview: Recombinant Mouse Gsk3b protein(Q9WV60)(Met1-Thr420), fused with His tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Mouse
• Tag: His
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 25% glycerol, 0.2mM DTT, pH 7.4Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. The specific activity was determined to be > 20 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate.2. Immobilized His-mGSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC50 of biotinylated human HG3C-CTNNB1 is 0.15-0.35 μg/ml.
• Molecular Mass: The recombinant mouse GSK3B consists of 439 amino acids and has a calculated molecular mass of 49 kDa. It migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
• Protein length: Met1-Thr420
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 75 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Gsk3b glycogen synthase kinase 3 beta [ Mus musculus ]
• Official Symbol: Gsk3b
• Synonyms: GSK3B; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK-3 beta; serine/threonine-protein kinase GSK3B; GSK3; GSK-3; C86142; GSK-3beta; 7330414F15Rik; 8430431H08Rik
• Gene ID: 56637
• mRNA Refseq: NM_019827
• Protein Refseq: NP_062801