Active Recombinant Mouse Growth Arrest Specific 1, His-tagged

• Cat.No.: Gas1-605M
• Product Overview: RecombinantMouse Gas1 protein was expressed in Murine myeloma cell line. Leu39-Ser315& Arg43-Ser315, both with a C-terminal 6-His tag.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 39-315;43-315 a.a.
• Description: Gas1 isone of six structurally unrelated proteins that were identified by theirincreased expression in growth-arrested cells relative to activelyproliferating cells. Following mitogenic stimulation, Gas1 expression is transcriptionallysuppressed by c-Myc as cells transit from G 0 to G1 phases of the cell cycle.Overexpression of Gas1 prevents S phase entry and DNA synthesis. Gas-mediatedblockade of the cell cycle is p53-dependent but does not require thetransactivating domain of p53. The mouse Gas1 cDNA encodes a 343 amino acid(aa) precursor that includes a 38 aa signal sequence, a 277 aa matureprotein, and a 28 aa C-terminal propeptide. Gas1 contains Ala-rich and Asp-richregions as well as an RGD sequence.
• Form: Lyophilized from a0.2 um filtered solution in PBS.
• N-terminalSequence: Leu39 & Arg43
• MolecularWeight: 29.8 kDa
• Activity: Measuredby its binding ability in a functional ELISA. Immobilized rhGas1 at 1 μg/mL(100 μL/well) can bind rmArtemin at a linear range of 0.1-10 ng/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 40 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at100μg/mL in PBS.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Gas1 growth arrest specific 1 [Mus musculus ]
• Official Symbol: Gas1
• Synonyms: Gas1; growth arrestspecific 1; Gas-1;AW554192; growth arrest-specific protein 1
• Gene ID: 14451
• mRNA Refseq: NM_008086
• Protein Refseq: NP_032112
• UniProt ID: Q01721
• Chromosome Location: 13
• Pathway: Hedgehog SignalingPathway
• Function: protein binding