Active Recombinant Mouse Granzyme G, His-tagged

• Cat.No.: Gzmg-1968M
• Product Overview: RecombinantMouse Gzmg protein was expressed in Spodoptera frugiperda. Ile21-Leu248,with a C-terminal 10-His tag.

Specification

• Cat. No.: Gzmg-1968M
• Description: Gzmg is amember of the Granzyme family of the serine proteases found specifically inthe cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer(NK) cells. Together with Gzmg, E and F, it isregulated through pregnancy and by IL-2 and IL-15 in granulated metrial glandcells. Human or rat counterpart of mouse Gzmg has notbeen found. Like other Granzymes, mouse Gzmg is notsecreted as a zymogen but stored as a fully processed and activated enzyme inthe cytoplasmic granules of CTL. It is synthesized as a 248 amino acid precursorwith a 18 amino acid signal peptide and a 2 amino acid propeptide. The matureprotein (residues 21-248) is expressed and purified.
• Source: Spodopterafrugiperda,Sf 21 (baculovirus) derived.
• Form: Supplied as a 0.2 umfiltered solution in Tris and NaCl.
• N-terminalSequence: Ile21
• MolecularWeight: 27 kDa
• Activity: Measuredby its ability to cleave a peptide substrate, Succinyl-Phe-Leu-Phe-ThioBenzylester (Suc-FLF-SBzl), in the presence of 5, 5'-Dithiobis (2-nitrobenzoic acid)(DTNB). The specific activity is > 150 pmoles/min/μg, as measured underthe described conditions.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 35 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70°C as supplied. 3 months, -20 to -70°C understerile conditions after opening.
• Protein length: 21-248 a.a.

Gene Information

• Gene Name: Gzmg granzyme G [ Musmusculus ]
• Official Symbol: Gzmg
• Synonyms: Gzmg; granzyme G; Ctla7;Ctla-7; MCSP-1; AKA granzyme G; CTL serine protease 1; CTL serine protease 3
• Gene ID: 14944
• mRNA Refseq: NM_010375
• Protein Refseq: NP_034505
• UniProt ID: P13366
• Chromosome Location: 14
• Pathway: Activation;Apoptosis; Intrinsic Pathway for Apoptosis
• Function: catalytic activity;hydrolase activity; peptidase activity; serine-type endopeptidase activity;serine-type peptidase activity