Active Recombinant Mouse Granzyme D, His-tagged

• Cat.No.: Gzmd-1967M
• Product Overview: RecombinantMouse Gzmd protein was expressed in Murine myeloma cell line. Ile21-Leu252,with a C-terminal 10-His tag.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 21-252 a.a.
• Description: Gzmd is amember of the granzyme family of the serine proteases found specifically inthe cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer(NK) cells. Gzmd'sfunctions are largely unknown. However, its selective expression in primaryand functional hematopoietic stromal lines correlates with capacity ofsupporting growth of a pre-T lymphoma clone. Together with Granzymes E, F andG, it is regulated through pregnancy and by IL-2 and IL-15 in granulatedmetrial gland cells. The human or rat counterpart of mouse Gzmd has notbeen found. Like other granzymes, mouse Gzmd is notsecreted as a zymogen but stored as a fully processed and activated enzyme inthe cytoplasmic granules of CTL. It is synthesized as a 252 amino acidprecursor with an 18 amino acid signal peptide and a 2 amino acid propeptide.The mature protein (residues 21-252) is expressed and purified.
• Form: Supplied as a 0.2 umfiltered solution in HEPES and NaCl.
• N-terminalSequence: Ile21 & His25
• MolecularWeight: 27 kDa
• Activity: Measuredby its ability to cleave a peptide substrate, Succinyl-Phe-Leu-Phe-ThioBenzylester (Suc-FLF-SBzl), in the presence of 5, 5'-Dithiobis (2-nitrobenzoic acid)(DTNB). The specific activity is > 50 pmoles/min/μg, as measured under thedescribed conditions.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 42 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100μg/mL in sterile 25 mM HEPES and 200 mM NaCl, pH 7.5.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70°C as supplied. 3 months, -20 to -70°C understerile conditions after opening.

Gene Information

• Gene Name: Gzmd granzyme D [ Musmusculus ]
• Official Symbol: Gzmd
• Synonyms: Gzmd; granzyme D; CCP5;Ctla5; Ctla-5
• Gene ID: 14941
• mRNA Refseq: NM_010372
• Protein Refseq: NP_034502
• UniProt ID: P11033
• Chromosome Location: 14
• Pathway: Activation;Apoptosis; Intrinsic Pathway for Apoptosis
• Function: hydrolase activity;peptidase activity; serine-type peptidase activity