Active Recombinant Mouse GP1BA Protein, C-His-tagged

• Cat.No.: GP1BA-21M
• Product Overview: Recombinant Mouse GP1BA Protein, fused to His-tag at C-terminus, was expressed in CHO.

Specification

• Species: Mouse
• Source: CHO
• Tag: His
• Protein Length: Gln17-Pro612
• Description: Acts upstream of or within blood coagulation and cell morphogenesis. Colocalizes with anchored component of external side of plasma membrane. Is expressed in dorsal root ganglion; embryo; liver; and spinal cord. Used to study Bernard-Soulier syndrome. Human ortholog(s) of this gene implicated in Bernard-Soulier syndrome; Bernard-Soulier syndrome type A2; myocardial infarction; non-arteritic anterior ischemic optic neuropathy; and platelet-type bleeding disorder 3. Orthologous to human GP1BA (glycoprotein Ib platelet subunit alpha).
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA. Recombinant Mouse CD42b/GPIb alpha is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human vWF‑A2 that produces 50% optimal binding response is approximately 0.1-0.5μg/mL in the presence of ristocetin.
• Molecular Mass: Predicted Molecular Mass: 66 kDa
  SDS-PAGE: 108-150 kDa, reducing conditions
• N-terminal Sequence Analysis: No results obtained: Gln17 inferred from enzymatic pyroglutamate treatment revealing His18
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Related Research Areas: Coagulation
  Myeloid Lineage Markers
  Platelet Adhesion Molecules
  Platelet Markers

Gene Information

• Gene Name: Gp1ba glycoprotein 1b, alpha polypeptide [ Mus musculus (house mouse) ]
• Official Symbol: GP1BA
• Synonyms: GPIba; GPIbalpha; GP-Ib alpha
• Gene ID: 14723
• mRNA Refseq: NM_010326.3
• Protein Refseq: NP_034456.2
• UniProt ID: O35930