Active Recombinant Mouse Glutathione S-Transferase, Mu 1, His-tagged

• Cat.No.: Gstm1-2507M
• Product Overview: Recombinant Gstm1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 28.1 kDa.

Specification

• Description: Gstm1 is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer ( pH8.0) containing 1mM DTT, 10% glycerol.
• Bio-activity: Specific activity is < 11 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (cdnb) with reduced glutathione per minute at ph 6.5 at
• Molecular Mass: 28.1 kDa (238aa), confirmed by MALDI-TOF
• Protein length: 1-218aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4? short term (1-2 weeks). For long term storage, aliquot and store at -20? or -70?. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: Gstm1 glutathione S-transferase, mu 1 [ Mus musculus ]
• Official Symbol: Gstm1
• Synonyms: GSTM1; glutathione S-transferase, mu 1; glutathione S-transferase Mu 1; pmGT10; GST 1-1; GST class-mu 1; glutathione S-transferase GT8.7; glutathione-S-transferase, mu 1; Gstb1; Gstb-1
• Gene ID: 14862
• mRNA Refseq: NM_010358
• Protein Refseq: NP_034488
• Pathway: Aflatoxin B1 metabolism, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Drug metabolism - cytochrome P450, organism-specific biosystem; Drug metabolism - cytochrome P450, conserved biosystem; Estrogen metabolism, organism-specific biosystem; Glutathione conjugation, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem
• Function: glutathione transferase activity; protein binding; protein homodimerization activity; steroid binding; transferase activity