Active Recombinant Mouse Gda Protein, His-tagged, Bioactivity Validated

• Cat.No.: Gda-219M
• Product Overview: Recombinant Mouse Gda(1-454aa) fused with His tag at N-terminal was expressed in E. coli. Bioactivity Validated.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: 1-454aa
• Description: Gda, also known as guanine deaminase, catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene.
• Form: Liquid. In 20mM Tris-HCl(pH8.0) containing 0.15M NaCl, 1mM DTT, 10% glycerol
• Bio-activity: Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.
• Molecular Mass: 53.4 kDa (477aa) Confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMCAARTP PLALVFRGTF VHSTWTCPME VLRDHLLGVS DSGKIVFLEE SSQQEKLAKE WCFKPCEIRE
  LSHHEFFMPG LVDTHIHAPQ YAFAGSNVDL PLLEWLNKYT FPTEQRFRST DVAEEVYTRV VRRTLKNGTT TACYFGTIHT DSSLILAEIT
  DKFGQRAFVG KVCMDLNDTV PEYKETTEES VKETERFVSE MLQKNYPRVK PIVTPRFTLS CTETLMSELG NIAKTHDLYI QSHISENREE
  IEAVKSLYPS YKNYTDVYDK NNLLTNKTVM AHGCYLSEEE LNIFSERGAS IAHCPNSNLS LSSGLLNVLE VLKHKVKIGL GTDVAGGYSY
  SMLDAIRRAV MVSNVLLINK VNEKNLTLKE VFRLATLGGS QALGLDSEIG NFEVGKEFDA LLINPRASDS PIDLFYGDFV GDISEAVIQK
  FLYLGDDRNI EEVYVGGKQV VPFSSSV
• Purity: > 95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280)

Gene Information

• Gene Name: Gda guanine deaminase [ Mus musculus ]
• Official Symbol: Gda
• Synonyms: GDA; guanine deaminase; GAH; guanase; guanine aminase; guanine aminohydrolase; AU015411; AW047581
• Gene ID: 14544
• mRNA Refseq: NM_010266
• Protein Refseq: NP_034396
• UniProt ID: Q9R111