Active Recombinant Mouse Furin (paired basic amino acid cleaving enzyme)

• Cat.No.: Furin-566M
• Product Overview: Recombinantmouse Furin protein was expressed in Chinese Hamster Ovary cell line, CHO-derived,with an N-terminus human CD33 signal peptide and a C-terminal 10-His tag. Gln25-Glu714.

Specification

• Cat. No.: Furin-566M
• Description: Furin is a member ofthe proprotein convertase (PC) family, which belongs to the subtilisinsuperfamily of serine proteases. As a cellular protease, Furin processes avariety of proproteins in secretory pathway compartments by cleaving after Arg-Xaa-Lys/Arg-Arg-like motifs, which usually reside at the end of the pro regions ofthese proproteins.
• Source: Chinese HamsterOvary cell line, CHO-derived
• Form: Supplied as a 0.2 μmfiltered solution in Tris, CaCl2, NaCl, Brij-35 and Glycerol.
• N-terminal Sequence: Asp108, Cys198,Ser239, Glu684
• Molecular Weight: 77 kDa
• Activity: Measured by itsability to cleave the fluorogenic peptide substrate pERTKR-AMC. The specificactivity is > 150 pmoles/min/μg, as measured under the described conditions.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• SDS-PAGE: 60-80 kDa, reducingconditions
• Purity: >95%, by SDS-PAGEunder reducing conditions and visualized by Colloidal Coomassie® Blue stainat 5 μg per lane.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3months, -20 to -70 °C under sterile conditions after reconstitution.
• Protein length: 25-714 a.a.

Gene Information

• Gene Name: Furin furin (paired basic aminoacid cleaving enzyme) [ Mus musculus ]
• Official Symbol: Furin
• Synonyms: Furin; furin (pairedbasic amino acid cleaving enzyme); OTTMUSP00000036135; OTTMUSP00000036136;OTTMUSP00000036137; OTTMUSP00000036138; Fur; PACE; SPC1; Pcsk3; 9130404I01Rik;prohormone convertase 3; diabasic-processing enzyme; substilisin pro-proteinprocessing enzyme; proprotein convertase substilisin/kexin type 3; pariedbasic amino acid residue cleaving enzyme; paried basic amino acidresidue-cleaving enzyme; EC 3.4.21.75
• Gene ID: 18550
• mRNA Refseq: NM_001081454
• Protein Refseq: NP_001074923
• UniProt ID: P23188
• Chromosome Location: 7
• Pathway: Delta-Notch Signaling Pathway;Gamma-carboxylation, transport, and amino-terminal cleavage of proteins;Influenza A; Maturation of Notch precursor via proteolytic cleavage;Metabolism of proteins; NGF processing; PTM: gamma carboxylation, hypusineformation and arylsulfatase activation; Post-translational proteinmodification; Removal of aminoterminal propeptides from gamma-carboxylatedproteins; Signal Transduction, Signaling by Notch; Signaling by PDGF; Signalingby TGF beta; Signalling by NGF
• Function: endopeptidase activity; hydrolase activity;metal ion binding; nerve growth factor binding; peptidase activity; peptidebinding; protease binding; protein binding; serine-type endopeptidaseactivity; serine-type endopeptidase inhibitor activity; serine-type peptidaseactivity