Active Recombinant Mouse Fst protein, His-tagged

• Cat.No.: Fst-6938M
• Product Overview: Recombinant Mouse Fst protein(P47931)(Met1-Trp344), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation.The ED50 for this effect is typically 0.02-0.08 μg/mL in the presence of 10 ng/ml Recombinant Human Activin A.
• Molecular Mass: The recombinant mouse FST comprises 326 amino acids and has a predicted molecular mass of 36.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFST is approximately 70-80 kDa due to glycosylation.
• Proteinlength: Met1-Trp344
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 1-344 a.a.

Gene Information

• Gene Name: Fst follistatin [ Mus musculus ]
• Official Symbol: Fst
• Synonyms: FST; follistatin; FS; activin-binding protein; AL033346
• Gene ID: 14313
• mRNA Refseq: NM_008046
• Protein Refseq: NP_032072