Active Recombinant Mouse FMS-like tyrosine kinase 1 Protein, Fc tagged

• Cat.No.: Flt1-12M
• Product Overview: Recombinant mouse VEGFR1/Flt-1, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: 23-759aa
• Description: Enables identical protein binding activity and vascular endothelial growth factor receptor activity. Involved in several processes, including blood vessel morphogenesis; embryonic morphogenesis; and hyaloid vascular plexus regression. Acts upstream of or within several processes, including angiogenesis; positive regulation of hematopoietic progenitor cell differentiation; and post-embryonic camera-type eye morphogenesis. Located in extracellular space and nucleus. Is expressed in several structures, including alimentary system; cardiovascular system; central nervous system; extraembryonic component; and genitourinary system. Human ortholog(s) of this gene implicated in Kuhnt-Junius degeneration; bronchopulmonary dysplasia; diabetic retinopathy; and pre-eclampsia. Orthologous to human FLT1 (fms related receptor tyrosine kinase 1).
• Tag: Fc
• Form: Liquid
• Molecular Mass: 108.9 kDa (970aa)
• AA Sequence: YGSGSKLKVPELSLKGTQHVMQAGQTLFLKCRGEAAHSWSLPTTVSQEDKRLSITPPSACGRDNRQFCSTLTLDTAQANHTGLYTCRYLPTSTSKKKKAESSIYIFVSDAGSPFIEMHTDIPKLVHMTEGRQLIIPCRVTSPNVTVTLKKFPFDTLTPDGQRITWDSRRGFIIANATYKEIGLLNCEATVNGHLYQTNYLTHRQTNTILDVQIRPPSPVRLLHGQTLVLNCTATTELNTRVQMSWNYPGKATKRASIRQRIDRSHSHNNVFHSVLKINNVESRDKGLYTCRVKSGSSFQSFNTSVHVYEKGFISVKHRKQPVQETTAGRRSYRLSMKVKAFPSPEIVWLKDGSPATLKSARYLVHGYSLIIKDVTTEDAGDYTILLGIKQSRLFKNLTATLIVNVKPQIYEKSVSSLPSPPLYPLGSRQVLTCTVYGIPRPTITWLWHPCHHNHSKERYDFCTENEESFILDPSSNLGNRIESISQRMTVIEGTNKTVSTLVVADSQTPGIYSCRAFNKIGTVERNIKFYVTDVPNGFHVSLEKMPAEGEDLKLSCVVNKFLYRDITWILLRTVNNRTMHHSISKQKMATTQDYSITLNLVIKNVSLEDSGTYACRARNIYTGEDILRKTEVLVRDSEAPHLLQNLSDYEVSISGSTTLDCQARGVPAPQITWFKNNHKIQQEPGIILGPGNSTLFIERVTEEDEGVYRCRATNQKGAVESAAYLTVQGTSDKSNLE
• Bio-Activity: Measured by its ability to inhibit proliferation using HUVEC human umbilical vein endothelial cells in the presence of Human VEGF165. The ED50 range ≤ 50 ng/mL.
• Endotoxin: < 1 EU/μg by LAL.
• Purity: > 85% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 1 mg/mL (determined by Absorbance at 280nm)
• Reference: 1. Peters, K.G. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8915-8919.
  2. Kendall R.L., et al., (1993), Natl. Acad. Sci. U.S.A. 90:10705-10709.
  3. Herley M.T., et al., (1999), Biochem. Biophys. Res. Commun. 262:731-738.

Gene Information

• Gene Name: Flt1 FMS-like tyrosine kinase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Flt1
• Synonyms: FLT1; FMS-like tyrosine kinase 1; vascular endothelial growth factor receptor 1; embryonic receptor kinase 2; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; vascular endothelial growth factor receptor-1; Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757
• Gene ID: 14254
• mRNA Refseq: NM_010228
• Protein Refseq: NP_034358
• UniProt ID: P35969