Active Recombinant Mouse Fibroblast Growth Factor Receptor 2, Fc-tagged

• Cat.No.: Fgfr2-542M
• Product Overview: Recombinant Mouse Fgfr2protein was expressed in Sf 21 with the Fc part of human IgG1 Pro100-Lys330(C-terminus) and Arg41-Glu308 (N-terminus).

Specification

• Cat. No.: Fgfr2-542M
• Description: Fibroblast growthfactors (FGFs) comprise a family of at least eighteen structurally relatedproteins that are involved in a multitude of physiological and pathologicalcellular processes, including cell growth, differentiation, angiogenesis,wound healing and tumorgenesis. The biological activities of the FGFs aremediated by a family of type I transmembrane tyrosine kinases which undergodimerization and autophosphorylation after ligand binding. Four distinctgenes encoding closely related FGF receptors, FGF R1-4, are known.
• Source: Spodopterafrugiperda, Sf 21 (baculovirus)-derived
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Arg41
• Molecular Weight: 56 kDa
• Activity: Measuredby its ability to inhibit FGF acidicdependent proliferation of NR6R-3T3 mousefibroblasts. The ED50 for this effect is typically 0.5-2 ng/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 74 kDa,reducing conditions
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100 μg/mL in sterile PBS
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt,-20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.
• Protein length: 41-308 a.a.

Gene Information

• Gene Name: Fgfr2 fibroblast growth factorreceptor 2 [ Mus musculus ]
• Official Symbol: Fgfr2
• Synonyms: Fgfr2; fibroblastgrowth factor receptor 2; Bek; svs; KGFR; Fgfr7; Fgfr-2; Fgfr-7; KGFRTr;AU043015; AW556123; fibroblast growth factor receptor 2; keratinocyte growthfactor receptor; EC 2.7.10.1; CD332; Ect1
• Gene ID: 14183
• mRNA Refseq: NM_010207
• Protein Refseq: NP_034337
• UniProt ID: P21803
• Chromosome Location: 7 F3; 7
• Pathway: Downstream signalingof activated FGFR; ESC Pluripotency Pathways; FGFR ligand binding andactivation; FGFR2 ligand binding and activation; IRS-mediated signalling; IRS-relatedevents; Insulin receptor signalling cascade; MAPK signaling pathway; PI-3Kcascade; Pathways in cancer; Phospholipase C-mediated cascade; Regulation ofActin Cytoskeleton; Endocytosis; Signaling by FGFR; Signaling by Insulinreceptor
• Function: ATP binding;fibroblast growth factor 1 binding; fibroblast growth factor binding;fibroblast growth factor-activated receptor activity; heparin binding; kinaseactivity; nucleotide binding; protein binding; protein homodimerizationactivity; protein kinase activity; protein tyrosine kinase activity; receptoractivity; transferase activity; transmembrane receptor protein tyrosinekinase activity