Active Recombinant Mouse Fibroblast Growth Factor 10

Cat. No. :	Fgf10-515M
Description :	TheFibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exerta variety of biological activities toward cells of mesenchymal, neuronal, andepithelial origin. Fgf10 belongs to the subgroup of FGFs that alsoincludes FGF-3, -7, and -22. Mature mouse Fgf10 is anapproximately 20 kDa protein that contains a serinerich region near itsNterminus2. C-terminal to this region (aa 62-209), it shares 94% and 100%amino acid sequence identity with human and rat Fgf10, respectively.Fgf10 issecreted by mesenchymal cells and associates with extracellular FGF-BP. Itpreferentially binds and activates epithelial cell FGF R2 (IIIb) andinteracts more weakly with FGF R1 (IIIb).
Source :	E. coli
Form :	Lyophilizedfrom a 0.2 um filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as acarrier protein.
N-terminalSequence :	Ser62
MolecularWeight :	17.1 kDa
Activity :	Measuredin a cell proliferation assay using 4MBr-5 monkey epithelial cells. The ED50 forthis effect is typically 20-120 ng/mL.
Endotoxin Level :	<1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE :	17 kDa,reducing conditions.
Purity :	>95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution :	Reconstituteat 100μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Storage :	Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.
Tag :	Non
Protein length :	62-209 a.a.

Gene Name	Fgf10fibroblast growth factor 10 [ Mus musculus ]
Official Symbol	Fgf10
Synonyms	Fgf10; fibroblastgrowth factor 10; Fgf-10; BB213776; keratinocyte growth factor 2
Gene ID	14165
mRNA Refseq	NM_008002
Protein Refseq	NP_032028
UniProt ID	O35565
Chromosome Location	13
Pathway	DevelopmentalBiology; Downstream signaling of activated FGFR; ESC Pluripotency Pathways; FGFRligand binding and activation; FGFR1 ligand binding and activation; FGFR1bligand binding and activation; FGFR2 ligand binding and activation; FGFR2bligand binding and activation; FRS2-mediated cascade; IRS-mediated signalling;IRS-related events; Insulin receptor signalling cascade; MAPK signalingpathway; Melanoma; PI-3K cascade
Function	chemoattractantactivity; fibroblast growth factor receptor binding; growth factor activity; heparinbinding; protein binding; type 2 fibroblast growth factor receptor binding