Active Recombinant Mouse Fgfr4 protein, His&hFc-tagged

• Cat.No.: Fgfr4-710M
• Product Overview: Recombinant Mouse Fgfr4 protein(NP_032037.2)(Met1-Asp366), fused with His and hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met1-Asp366
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit FGF acidic (aFGF / FGF1) dependent proliferation of Balb/c3T3 mouse embryonic fibroblasts .The ED50 for this effect is typically 0.1-0.6 μg/mL.
• Molecular Mass: The recombinant mouse FGFR4/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 598 amino acids and has a predicted molecular mass of 67 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FGFR4/Fc monomer is approximately 100-110 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fgfr4 fibroblast growth factor receptor 4 [ Mus musculus ]
• Official Symbol: Fgfr4
• Synonyms: FGFR4; fibroblast growth factor receptor 4; CTLA-2-beta protein; protein-tyrosine kinase receptor MPK-11; fibroblast growth factor receptor 4 16 minus form; Fgfr-4
• Gene ID: 14186
• mRNA Refseq: NM_008011
• Protein Refseq: NP_032037