Active Recombinant Mouse Fcgr1 Protein, His-tagged, Biotinylated

• Cat.No.: Fcgr1-182M
• Product Overview: Biotinylated Mouse Fc gamma RI / CD64 Protein (CD4-M52E8) is expressed from human HEK293 cells. It contains AA Glu 25 - Pro 297 (Accession # NP_034316).

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% Trehalose.
• Bio-activity: The bioactivity is measured by its binding ability to mouse IgG2a in a SPR assay.Immobilized Biotinylated Mouse Fc gamma RI / CD64 Protein can bind mouse IgG2a with affinity constant of around 6.0 nM.Measured by its binding ability in a functional ELISA.Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse Fc gamma RI with a linear range of 0.03-0.5 μg/mL.
• Molecular Mass: This protein carries a 15 AA fragment at the C-terminus, followed by a polyhistidine tag. There is one single lysine residue within the 15 AA fragment that is enzymatically labeled with biotin in vivo. The protein has a calculated MW of 33 kDa. The predicted N-terminus is Glu 25. The reducing (R) protein migrates as 45-60 kDa in SDS-PAGE due to glycosylation.
• Protein length: Glu 25 - Pro 297
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Fcgr1 Fc receptor, IgG, high affinity I [ Mus musculus (house mouse) ]
• Official Symbol: Fcgr1
• Synonyms: Fcgr1; Fc receptor, IgG, high affinity I; high affinity immunoglobulin gamma Fc receptor I; IgG high affinity Fc receptor; fc-gamma RI; fcRI; igG Fc receptor I
• Gene ID: 14129
• mRNA Refseq: NM_010186.5
• Protein Refseq: NP_034316.1
• UniProt ID: P26151