Active Recombinant Mouse Fbln7 Protein (Gln25-Phe440), N-HA tagged

• Cat.No.: Fbln7-12M
• Product Overview: Recombinant Mouse Fibulin 7 Protein (Gln25-Phe440) with an N-terminal HA Tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Species: Mouse
• Source: CHO
• Tag: HA
• Protein Length: Gln25-Phe440
• Description: Enables heparan sulfate proteoglycan binding activity and heparin binding activity. Acts upstream of or within positive regulation of biomineralization. Located in extracellular matrix and extracellular space. Is expressed in several structures, including alimentary system; brain; reproductive system; respiratory system; and skeleton. Orthologous to human FBLN7 (fibulin 7).
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. The ED50 for this effect is 0.7-3.5 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 47 kDa
  SDS-PAGE: 53-63 kDa, reducing conditions
• N-terminal Sequence Analysis: Tyr
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE with silver staining
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: Fbln7 fibulin 7 [ Mus musculus (house mouse) ]
• Official Symbol: Fbln7
• Synonyms: Fbln7; fibulin 7; TM14; 1600015H20Rik; fibulin-7; FIBL-7; extracellular matrix protein TM14
• Gene ID: 70370
• mRNA Refseq: NM_024237
• Protein Refseq: NP_077199
• UniProt ID: Q501P1