Active Recombinant Mouse Fasl protein, HA-tagged

• Cat.No.: Fasl-482M
• Product Overview: Recombinant Mouse Fasl(Gln101-Leu279) fused with HA tag at N-terminal was expressed in CHO.

Specification

• Source: CHO
• Species: Mouse
• Tag: HA
• Predicted N Terminal: Tyr
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 1-8 ng/mL in the presence of 2.5 µg/mL of a cross-linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody.
• Molecular Mass: Predicted Molecular Mass: 25.9 (monomer) kDa;
  SDS-PAGE: 40-55 kDa, reducing conditions.
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
• Protein length: 101-279 a.a.

Gene Information

• Gene Name: Fasl Fas ligand (TNF superfamily, member 6) [ Mus musculus ]
• Official Symbol: Fasl
• Synonyms: FASL; Fas ligand (TNF superfamily, member 6); tumor necrosis factor ligand superfamily member 6; CD95 ligand; Fas antigen ligand; generalized lymphoproliferative disease; gld; CD178; CD95L; Fas-L; Faslg; CD95-L; Tnfsf6; APT1LG1
• Gene ID: 14103
• mRNA Refseq: NM_001205243
• Protein Refseq: NP_001192172
• UniProt ID: P41047