Active Recombinant Mouse Epyc Protein, His-tagged

• Cat.No.: Epyc-594M
• Product Overview: Recombinant Mouse Epyc (Accession # NP_031910) Ala20-Ile322, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ala20
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to inhibit the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin.When 5 x 10^4 cells/well are added to Mouse DSPG3 and Human Fibronectin (0.5 µg/mL) coated plates, the cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is typically 0.3 - 1.2 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Epyc, His-tagged has a calculated MW of 36.2 kDa. In SDS-PAGE migrates as 52-57 kDa, reducing conditions.
• Protein length: Ala20-Ile322
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Epyc epiphycan [ Mus musculus (house mouse) ]
• Official Symbol: Epyc
• Synonyms: Dspg3; PG-Lb; SLRR3B; epiphycan; epiphycan; dermatan sulfate proteoglycan 3; dermatan sulphate proteoglycan 3; proteoglycan-Lb; small chondroitin/dermatan sulfate proteoglycan
• Gene ID: 13516
• mRNA Refseq: NM_007884.2
• Protein Refseq: NP_031910.1
• UniProt ID: P70186
• Chromosome Location: 10 50.35 cM; 10 C2-C3