Active Recombinant Mouse Epo protein, His-tagged

• Cat.No.: Epo-7421M
• Product Overview: Recombinant Mouse Epo protein(P07321)(Met1-Arg192), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Arg192
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind biotinylated human EPOR-His in a functional ELISA.2. Measured by its ability to bind mouse EPOR-Fch in a functional ELISA.3. Measured by its ability to bind human EPOR & CD131-Fc in a functional ELISA.4. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 2-10 ng/mL.
• Molecular Mass: The recombinant mouse EPO comprises 177 amino acids and has a predicted molecular mass of 20 kDa. The apparent molecular mass of the protein is approximately 37 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Epo erythropoietin [ Mus musculus ]
• Official Symbol: Epo
• Synonyms: EPO; erythropoietin
• Gene ID: 13856
• mRNA Refseq: NM_007942
• Protein Refseq: NP_031968