Active Recombinant Mouse Epithelial Mitogen

• Cat.No.: Epgn-455M
• Product Overview: Recombinant Mouse Epgnprotein was expressed in E. coli. Leu53-Ala103.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Protein Length: 53-103 a.a.
• Description: Epgn is aneipthelial mitogen that belongs to the EGF superfamily. Mouse Epgn cDNA encodesa 153 amino acid (aa) residue type I transmembrane precursor protein with a18 aa putative signal peptide, a 92 aa extracellular domain, a 20 aa transmembranedomain and a 22 aa cytoplasmic domain. Expression of mouse Epgn has beendetected in multiple tissues including testis, liver and heart. Over theentire protein, mouse Epgn shares fromapproximately 23% to 33% sequence homology with other EGF family members. Thefunctional internal peptide containing the EGF motif however is more than 40%homologous with that of TGFα and epiregulin. A human cDNA clone encoding amouse epigenlike protein has also been identified.
• Form: Lyophilizedfrom a 0.2 um filtered solution in PBS with BSA as a carrier protein.
• N-terminalSequence: Leu53
• MolecularWeight: 5.9 kDa
• Activity: Measuredin a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. TheED50 for this effect is typically 50 150 ng/mL.
• Endotoxin Level: <1.0EU per 1 μg of the protein by the LAL method.
• Purity: >97%,by SDSPAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100μg/mL in sterile PBS containing at least 0.1% human or bovine serumalbumin.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, 20 to 70°C as supplied. 1 month,2 to 8°C under sterile conditions after reconstitution. 3 months, 20 to 70°Cunder sterile conditions after reconstitution.

Gene Information

• Gene Name: Epgn epithelial mitogen [ Musmusculus ]
• Official Symbol: Epgn
• Synonyms: Epgn; epithelialmitogen; epigen;2310069M11Rik; epigen; EPG
• Gene ID: 71920
• mRNA Refseq: NM_053087
• Protein Refseq: NP_444317
• UniProt ID: Q0VEB7
• Chromosome Location: 5
• Function: MAP kinase kinaseactivity; epidermal growth factor receptor binding; growth factor activity