Active Recombinant Mouse EphA4 protein, hFc-tagged

• Cat.No.: Epha4-912M
• Product Overview: Recombinant Mouse EphA4 protein(NP_031962.2)(Met1-Thr547), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Thr547
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse EPHA5 at 2 μg/ml (100 μl/well) can bind mouse EFNA4-Fc with a linear range of 1.28-32 ng/ml.
• Molecular Mass: The recombinant mouse EPHA4/Fc is a disulfide-linked homodimer. The reduced monomer consists of 768 amino acids and has a predicted molecular mass of 85.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEPHA4/Fc monomer is approximately 110 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Epha4 Eph receptor A4 [ Mus musculus ]
• Official Symbol: EphA4
• Synonyms: EPHA4; Eph receptor A4; ephrin type-A receptor 4; rabbit; snoRNA MBII-267; tyrosine-protein kinase receptor MPK-3; tyrosine-protein kinase receptor SEK-1; rb; Sek; Cek8; Hek8; Sek1; Tyro1; AI385584; 2900005C20Rik
• Gene ID: 13838
• mRNA Refseq: NM_007936
• Protein Refseq: NP_031962