Active Recombinant Mouse Epha1 protein, His-tagged

• Cat.No.: Epha1-6923M
• Product Overview: Recombinant Mouse Epha1 protein(Q60750)(Met1-Glu548), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse EPHA1-His at 10 μg/ml (100 μl/well) can bind mouse EFNA1-Fc, The EC50 of mouse EFNA1-Fc is 21.3-49.8 ng/ml.
• Molecular Mass: The secreted recombinant mouse EPHA1 comprises 535 amino acids and has a calculated molecular mass of 58.6 kDa. As a result of glycosylation, the apparent molecular mass of rmEPHA1 is approximately 68 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met1-Glu548
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Epha1 Eph receptor A1 [ Mus musculus ]
• Official Symbol: Epha1
• Synonyms: EPHA1; Eph receptor A1; ephrin type-A receptor 1; mEpha1; embryonic stem cell kinase; tyrosine-protein kinase receptor ESK; Eph; Esk; AL033318; 5730453L17Rik
• Gene ID: 13835
• mRNA Refseq: NM_023580
• Protein Refseq: NP_076069