Active Recombinant Mouse Eng protein, His-tagged

• Cat.No.: Eng-3289M
• Product Overview: Recombinant Mouse Eng protein(NP_031958.2)(Met1-Gly580), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. The ED50 for this effect is typically 25-100 ng/mL in the presence of 2 ng/mL of recombinant human BMP9.
• Molecular Mass: The secreted recombinant mouse CD105 consists of 565 amino acids and has a calculated molecular mass of 61.2 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 65-70 kDa in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-Gly580
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 1-580 a.a.

Gene Information

• Gene Name: Eng endoglin [ Mus musculus ]
• Official Symbol: Eng
• Synonyms: ENG; endoglin; transmembrane glycoprotein; cell surface MJ7/18 antigen; CD105; AI528660; AI662476; S-endoglin
• Gene ID: 13805
• mRNA Refseq: NM_001146348
• Protein Refseq: NP_001139820