Active Recombinant Mouse Ecm1 protein, His-tagged

• Cat.No.: Ecm1-4027M
• Product Overview: Recombinant Mouse Ecm1 protein(Q61508-1)(Met1-Glu559), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-559 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of HFF Human skin fibroblast cells. When 5 × 10E4 cells/well are added to mECM1-His coated plates (2.5 μg/mL and 100 μL/well), approximately > 45 % will adhere specifically after 90 minutes at 37 ℃.
• Molecular Mass: The secreted recombinant mouse ECM1 comprises 551 amino acids and has a predicted molecular mass of 62.5 kDa. As a result of glycosylation, the apparent molecular mass of rm ECM1 is approximately 90-95 kDa in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-Glu559
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 88 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ecm1 extracellular matrix protein 1 [ Mus musculus ]
• Official Symbol: Ecm1
• Synonyms: ECM1; extracellular matrix protein 1; secretory component p85; p85; AI663821
• Gene ID: 13601
• mRNA Refseq: NM_001252653
• Protein Refseq: NP_001239582