Active Recombinant Mouse Dll4, His-tagged

• Cat.No.: Dll4-451M
• Product Overview: Recombinant Mouse Dll4 (Accession # BAB18580) Ser28-Pro525, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 28-525 a.a.
• Predicted N Terminal: Ser28
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842.The ED50 for this effect is typically 150-600 ng/mL.
• Molecular Mass: Recombinant Mouse Dll4, His-tagged has a calculated MW of 55.6 kDa. In SDS-PAGE migrates as 75-80 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 200 µg/mL in sterile PBS.

Gene Information

• Gene Name: Dll4 delta-like 4 (Drosophila) [ Mus musculus ]
• Official Symbol: Dll4
• Synonyms: DLL4; delta-like 4 (Drosophila); delta-like protein 4; drosophila Delta homolog 4; Delta4
• Gene ID: 54485
• mRNA Refseq: NM_019454
• Protein Refseq: NP_062327