Active Recombinant Mouse Dll1, His tagged

• Cat.No.: Dll1-4036M
• Product Overview: Recombinant Mouse Dll1 (NP_031891.2) extracellular domain (Met 1-Gln 516), fused with a polyhistidine tag at the C-terminus, was produced in Human Cell.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met 1-Gln 516
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind human NOTCH1 in a functional ELISA.
  2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-20 μg/mL.
• Molecular Mass: The recombinant mouse DLL1 consists of 506 amino acids and has a predicted molecular mass of 55 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm DLL1 is approximately 65 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Dll1 delta-like 1 (Drosophila) [ Mus musculus ]
• Official Symbol: Dll1
• Synonyms: DLL1; delta-like 1 (Drosophila); delta-like protein 1; delta like-1; drosophila Delta homolog 1; Delta1
• Gene ID: 13388
• mRNA Refseq: NM_007865
• Protein Refseq: NP_031891