Active Recombinant Mouse Dipeptidylpeptidase 7, His-tagged

• Cat.No.: Dpp7-416M
• Product Overview: Recombinantmouse Dpp7 protein was expressed in Murine myeloma cell line, NS0-derived, with a C-terminal10-His tag.Asp34-Arg506

Specification

• Cat. No.: Dpp7-416M
• Description: The protein encodedby this gene is a post-proline cleaving aminopeptidase expressed in quiescentlymphocytes. The resting lymphocytes are maintained through suppression ofapoptosis, a state which is disrupted by inhibition of this novel serineprotease. The enzyme has strong sequence homology with prolylcarboxypeptidaseand is active at both acidic and neutral pH.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in Tris and Nacl.
• N-terminal Sequence: Asp34
• Molecular Weight: 54 kDa
• Activity: Measured by itsability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin(GP-AMC). The specific activity is > 2,500 pmoles/min/μg, as measuredunder the described conditions. See Activity Assay Protocol.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 200μg/mL in 25 mM Tris and 150 mM NaCl, pH 7.5.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3months, -20 to -70 °C under sterile conditions after reconstitution.
• Protein length: 34-506 a.a.

Gene Information

• Gene Name: Dpp7dipeptidylpeptidase 7 [ Mus musculus ]
• Official Symbol: Dpp7
• Synonyms: Dpp7;dipeptidylpeptidase 7; QPP; Dpp2; DPPII; OTTMUSP00000012875; dipeptidylpeptidase 2; dipeptidyl-peptidase 2; dipeptidyl peptidase II; dipeptidyl-peptidaseII; dipeptidyl aminopeptidase II; quiescent cell proline dipeptidase;Ec 3.4.14.2; dipeptidylpeptidase 7
• Gene ID: 83768
• mRNA Refseq: NM_031843
• Protein Refseq: NP_114031
• UniProt ID: Q9ET22
• Chromosome Location: 2
• Function: aminopeptidase activity;dipeptidyl-peptidase activity; hydrolase activity; peptidase activity;serine-type peptidase activity