Active Recombinant Mouse Dickkopf Homolog 2 (Xenopus laevis)

• Cat.No.: Dkk2-401M
• Product Overview: Recombinantmouse Dkk2 protein was expressed in Spodoptera frugiperda, Sf 21(baculovirus)-derived. Ser26-Ile259, with a C-terminal10-His tag.

Specification

• Cat. No.: Dkk2-401M
• Description: Dkk2 is a member ofthe Dickkopf family of secreted Wnt modulators. Dkk proteins contain a signalpeptide and two conserved cysteine-rich domains that are separated by alinker region. The second cysteine-rich domain mediates Dkk2 bindingactivities, and its interaction with LRP beta propellers has been mapped.
• Source: Spodopterafrugiperda, Sf 21 (baculovirus)-derived
• Form: Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein.
• N-terminal Sequence: Ser26
• Molecular Weight: 27.1 kDa
• Activity: Measured by itsability to inhibit Wnt-3a-induced alkaline phosphates production in thepresence of BMP2 by MC3T3-E1 mouse osteoblastic cells. Measured in acompetitive binding assay. When Recombinant Mouse Kremen1 is immobilized at 4μg/mL (100 μL/well), Recombinant Mouse Dkk2 inhibits 50% binding ofbiotinylated Recombinant Human Dkk1 (200 ng/mL) at the concentration range of2-8 μg/mL.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 100μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.
• Protein length: 26-259 a.a.

Gene Information

• Gene Name: Dkk2dickkopf homolog 2 (Xenopus laevis) [ Mus musculus ]
• Official Symbol: Dkk2
• Synonyms: Dkk2; dickkopfhomolog 2 (Xenopus laevis); dickkopf-related protein 2; dkk-2; mDkk-2;dickkopf 2; dickkopf-2; dickkopf homolog 1; mCG_50274
• Gene ID: 56811
• mRNA Refseq: NM_020265
• Protein Refseq: NP_064661
• UniProt ID: Q8BFW0
• Chromosome Location: 3
• Pathway: Wntsignaling pathway