Active Recombinant Mouse Dickkopf Homolog 1 (Xenopus laevis), His-tagged

• Cat.No.: Dkk1-395M
• Product Overview: Recombinantmouse Dkk1 protein was expressed in Murine myeloma cell line, NS0-derived, with a C-terminal10-His tag. Ser30-His272.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 30-272 a.a.
• Description: Dkk1 is the foundingmember of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, anda related protein, Soggy. Dkk proteins are secreted proteins that contain twoconserved cysteine-rich domains separated by a linker region. Each domaincontains ten cysteine residues. Mature mouse Dkk1 is a 40 kDa glycosylatedprotein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identitywith human, rat, rabbit and bovine Dkk1, respectively.
• Form: Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein.
• N-terminal Sequence: Ser30
• Molecular Weight: 26.1 kDa
• Activity: Measured by itsability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3E1 mouseosteoblastic cells. The ND50 for this effect is typically 0.2-1 μg/mLin the presence of 10 ng/mL of rmWnt-3a. Measured by its ability to interferewith the axisinducing activity of Xwnt-8 mRNA in early Xenopusembryos.In a population of embryos, the addition of 1 ng of rmDkk1 per embryoresulted in 60-100% reduction in the number of secondary axes produced by 10-20pg of Xwnt-8 mRNA. Optimal concentrations should be determined by eachlaboratory for each application.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 100μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Dkk1dickkopf homolog 1 (Xenopus laevis) [ Mus musculus ]
• Official Symbol: Dkk1
• Synonyms: Dkk1; dickkopfhomolog 1; mddk-1; OTTMUSP00000022079; dickkopf-related protein 1; dkk-1; dickkopf-1;mCG_6758
• Gene ID: 13380
• mRNA Refseq: NM_010051
• Protein Refseq: NP_034181
• UniProt ID: Q80UL5
• Chromosome Location: 19
• Pathway: Wnt SignalingPathway; PluriNetWork
• Function: Low-density lipoprotein particle binding; receptorantagonist activity; protein binding