Active Recombinant Mouse Cysteine-Rich Secretory Protein 4, His-tagged
Cat.No. : | Crisp4-5612M |
Product Overview : | Recombinant Mouse Crisp4protein was expressed in CHO with a C-terminal 6-His tag. Pro22-Lys250. |
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Cat. No. : | Crisp4-5612M |
Description : | Crisp4 is one ofseveral CRISPs (cysteine-rich secretory proteins) that contain 16 invariantcysteine residues and show structural homolgy to snake, insect, and lizardvenom proteins. These proteins consist of an N-terminal SCP/CAP domain, ahinge region, and a cysteine-rich CRISP domain. CRISPs are expressed indiscrete but overlapping regions of the male reproductive tract and play arole in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation,and the acrosomal reaction. |
Source : | Chinese HamsterOvary cell line, CHO-derived |
Form : | Lyophilized from a0.2 μm filtered solution in PBS. |
N-terminalSequence : | Leu23 |
Molecular Weight : | 27.2 kDa |
Activity : | Measuredby the ability of the immobilized protein to support the adhesion of NTera-2human testicular embryonic carcinoma cells. The ED50 for thiseffect is typically 0.3-1.5 μg/mL after 1 hour incubation at 37 °C. Optimaldilutions should be determined by each laboratory for each application. |
Endotoxin Level : | <1.0 EUper 1 μg of the protein by the LAL method. |
SDS-PAGE : | 34-37kDa, reducing conditions |
Purity : | >95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
Reconstitution : | Reconstituteat 200 μg/mL in PBS |
Storage : | Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution. |
Protein length : | 22-250 a.a. |
Gene Name | Crisp4 cysteine-rich secretoryprotein 4 [ Mus musculus ] |
Official Symbol | Crisp4 |
Synonyms | Crisp4; cysteine-richsecretory protein 4; 9230112K08Rik; cysteine-rich secretory protein 1;Uncharacterized protein |
Gene ID | 78081 |
mRNA Refseq | NM_030033 |
Protein Refseq | NP_084309 |
UniProt ID | Q9D259 |
Chromosome Location | 1 A3; 1 |
Function | molecular function |
Not For Human Consumption!
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