Active Recombinant Mouse Cpa1 protein, His-tagged

• Cat.No.: Cpa1-2287M
• Product Overview: Recombinant Mouse Cpa1 protein(NP_079626.2)(Met1-Tyr419), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5'Dithiobis (2-nitrobenzoic acid) (DTNB). The specific activity is >6,000 pmoles/min/μg.
• Molecular Mass: The secreted recombinant pro form of mouse CPA1 comprises 414 amino acids and has a calculated molecular mass of 47 kDa. The recombinant protein migrates as an approximately 42 kDa band in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-Tyr419
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 1-419 a.a.

Gene Information

• Gene Name: Cpa1 carboxypeptidase A1, pancreatic [ Mus musculus ]
• Official Symbol: Cpa1
• Synonyms: CPA1; carboxypeptidase A1, pancreatic; carboxypeptidase A1; Cpa; 0910001L12Rik
• Gene ID: 109697
• mRNA Refseq: NM_025350
• Protein Refseq: NP_079626