Active Recombinant Mouse Contactin 6, His-tagged

• Cat.No.: Cntn6-462M
• Product Overview: Recombinant Mouse Cntn6protein was expressed in CHO using a C-terminal 6-His tag. Glu20-Ser998.

Specification

• Cat. No.: Cntn6-462M
• Description: Cntn6, also known asNB3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPIlinked neuronaladhesion proteins. Mature mouse Cntn6 consists of six immunoglobulinlike domains,a flexible linker region, four fibronectin type III domains, and a GPI anchor.Mouse Cntn6 shares 90% and 96% amino acid (aa) sequence identity with human andrat Cntn6, respectively. Alternate splicing generates an isoform with a 17 aadeletion in the first Iglike domain.
• Source: Chinese Hamster Ovarycell line, CHO-derived
• Form: Lyophilized from a 0.2μm filtered solution in PBS.
• N-terminalSequence: Glu20
• Molecular Weight: 109.2 kDa
• Activity: Measuredby the ability of the immobilized protein to support the adhesion of Neuro2a mouseneuroblastoma cells (ATCC: CCL131). When 5 x 104 cells/well are addedto rmCntn6 coated plates (10 μg/mL, 100 μL/well), approximately 40-60% will adhereafter 30 minutes at 37 °C. Optimal dilutions should be determined by each laboratoryfor each application.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100 μg/mL in sterile PBS
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.
• Protein length: 20-998 a.a.

Gene Information

• Gene Name: Cntn6 contactin6 [ Mus musculus ]
• Official Symbol: Cntn6
• Synonyms: Cntn6; contactin6; NB-3; mNB-3; neural recognition molecule NB-3
• Gene ID: 53870
• mRNA Refseq: NM_017383
• Protein Refseq: NP_059079
• UniProt ID: Q9JMB8
• Chromosome Location: 6; 6 E2