Active Recombinant Mouse Ces2e protein, His-tagged

• Cat.No.: Ces2e-913M
• Product Overview: Recombinant Mouse Ces2e protein(NP_766347.1)(Met1-His556), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile 25mM Tris, 150mM NaCl, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to hydrolyze pnitrophenylacetate. The specific activity is >50,000 pmoles/min/μg.
• Molecular Mass: The secreted recombinant mouse CES5 comprises 541 amino acids and has a calculated molecular mass of 60.6 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 52 kDa band in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-His556
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 88 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 1-556 a.a.

Gene Information

• Gene Name: Ces2e carboxylesterase 2E [ Mus musculus ]
• Official Symbol: Ces2e
• Synonyms: CES2E; carboxylesterase 2E; carboxylesterase 5; Ces5; 9030624L02Rik
• Gene ID: 234673
• mRNA Refseq: NM_001163756
• Protein Refseq: NP_001157228