Active Recombinant Mouse Cdh2 Protein, Fc Chimera

• Cat.No.: Cdh2-592M
• Product Overview: Recombinant Mouse Cdh2 (Accession # NP_031690), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc
• Predicted N Terminal: Asp160, Ser26, & Glu28
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U-118-MG human glioblastoma/astrocytoma cells.The ED50 for this effect is typically 0.1 - 0.5 µg/mL.
• Molecular Mass: Recombinant Mouse Cdh2, Fc Chimera has a calculated MW of 88.8 kDa (monomer, mature protein) & 104.2 kDa (monomer, pro-protein). In SDS-PAGE migrates as (115-120) kDa & (130-135) kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Cdh2 cadherin 2 [ Mus musculus ]
• Official Symbol: Cdh2
• Synonyms: CDH2; cadherin 2; cadherin-2; neural cadherin; CDHN; Ncad; N-cadherin
• Gene ID: 12558
• mRNA Refseq: NM_007664
• Protein Refseq: NP_031690