Active Recombinant Mouse Cd70 Protein (Gln47-Pro195), N-His tagged, Animal-free, Carrier-free

• Cat.No.: Cd70-87M
• Product Overview: Recombinant Mouse Cd70 Protein (Gln47-Pro195) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: CD27L is also named for CD70 which is one member of TNF family. CD27L is expressed on T and B lymphocytes and mature DCs. It binds the CD27, which is on the antigen-presenting cells. CD27L is a 19.2 kDa cytokine with 148 amino acid residues which is a transmembrane glycoprotein. CD27L plays an important role in the regulation of T cell proliferation which is also a good target of cancer immunotherapy.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in mouse T cells in the presence of the anti-CD3 antibody. The ED50 for this effect is < 4.5 μg/mL
• Molecular Mass: The protein has a calculated MW of 17.25 kDa.
  The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Gln47-Pro195
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Cd70 CD70 antigen [ Mus musculus (house mouse) ]
• Official Symbol: Cd70
• Synonyms: CD70; CD70 antigen; CD27-L; CD27 ligand; Ki-24 antigen; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7; Cd27l; CD27LG; Tnfsf7
• Gene ID: 21948
• mRNA Refseq: NM_011617
• Protein Refseq: NP_035747
• UniProt ID: O55237