Active Recombinant Mouse CD48 Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD48-867MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant mouse CD48 (NP_031675.1) without the propeptide, corresponding to the amino acids (Met 1-Arg 216) was expressed with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Bio-activity: 1. Measured by its ability to bind human 2B4 in a functional ELISA.
  2. Measured by its ability to bind biotinylated recombinant mouse CD2 in a functional ELISA.
• Molecular Mass: The secreted recombinant mouse CD48 consists of 205 amino acids and has a calculated molecular mass of 23.7 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 45-60 kDa in SDS-PAGE under reducing conditions.
• Protein length: 205
• N-terminal Sequence Analysis: Phe 23
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Cd48 CD48 antigen [ Mus musculus ]
• Official Symbol: CD48
• Synonyms: CD48; CD48 antigen; HM48-1; BCM1 surface antigen; MRC OX-45 surface antigen; BCM1; BLAST; Bcm-1; BLAST1; SLAMF2; Sgp-60; BLAST-1; MEM-102; AI449234; AW610730
• Gene ID: 12506
• mRNA Refseq: NM_007649
• Protein Refseq: NP_031675