Active Recombinant Mouse CD200 protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: CD200-3071M
• Product Overview: Biotinylated Recombinant Mouse CD200 protein(Gln 31-Gly 232), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: Gln 31-Gly 232
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD200 R1, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse CD200, Fc,Avitag with a linear range of 0.02-0.5 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 50.5 kDa. The protein migrates as 66-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd200 CD200 antigen [ Mus musculus ]
• Official Symbol: CD200
• Synonyms: CD200; CD200 antigen; OX-2 membrane glycoprotein; MRC OX-2 antigen; antigen identified by monoclonal MRC OX-2; OX2; Mox2
• Gene ID: 17470
• mRNA Refseq: NM_010818
• Protein Refseq: NP_034948