Active Recombinant Mouse Cd14 Protein, Fc-tagged

• Cat.No.: Cd14-117M
• Product Overview: Recombinant mouse Cd14 (Ala18­Pro345) protein with Fc region of human IgG1 at the C-terminus was expressed in mouse myeloma cell line, NS0.

Specification

• Description: This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor.
• Source: Mammalian cells
• Species: Mouse
• Tag: Fc
• Bio-activity: Measured by its ability to enhance LPS-induced IL-6 secretion by mouse splenocytes. The ED50 for this effect is 0.4-2.4 µg/mL.
• Molecular Mass: Predicted Molecular Mass: 61.8 kDa (monomer)
  SDS-PAGE: 85-95 kDa, reducing conditions
• N-terminal Sequence Analysis: Ala18
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 1 mg/mL in sterile PBS.

Gene Information

• Gene Name: Cd14
• Official Symbol: Cd14 CD14 antigen [ Mus musculus (house mouse) ]
• Synonyms: Cd14; CD14 antigen; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
• Gene ID: 12475
• mRNA Refseq: NM_009841
• Protein Refseq: NP_033971
• UniProt ID: P10810