Active Recombinant Mouse Car10, His-tagged

• Cat.No.: Car10-1805M
• Product Overview: A DNA sequence encoding the mouse Car10 (Met 1-Asn 300) was expressed, fused with a C-terminal polyhistidine tag.

Specification

• Description: Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA10, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.
• Source: Human Cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Gln 23
• Form: Lyophilized from sterile 25mM Tris, 150mM NaCl, pH 7.4
• Bio-activity: Measured by its esterase activity. The specific activity is >10 pmoles/min/μg.
• Molecular Mass: The secreted recombinant mouse Car10 comprises 289 amino acids and has a calculated molecular mass of 33.1 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 38 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >97 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products.?
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Protein length: 1-300 a.a.

Gene Information

• Gene Name: Car10?carbonic anhydrase 10 [?Mus musculus?(house mouse) ]
• Official Symbol: Car10
• Synonyms: Car10; Ca10; BB085816; 2700029L05Rik; carbonic anhydrase 10; carbonic anhydrase-related protein 10; CARP X; CA-RP X; carbonic anhydrase-related protein X
• Gene ID: 72605
• mRNA Refseq: NM_028296
• Protein Refseq: NP_082572
• UniProt ID: P61215
• Chromosome Location: 11; 11 C
• Function: molecular_function