Active Recombinant Mouse C5a protein, His-tagged

• Cat.No.: C5a-2999M
• Product Overview: Recombinant Mouse C5a protein(Asn 679-Arg 755), fused with His tag, was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Asn 679-Arg 755
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Mouse Complement C5a, His Tag at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.4-6 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 10.9 kDa. The protein migrates as 14 kDa under reducing (R) condition (SDS-PAGE).
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.