Active Recombinant Mouse Bmper, His-tagged

• Cat.No.: Bmper-344M
• Product Overview: Recombinant Mouse Bmper (Accession # AAH66153) Val34-Arg685, Ala39-Asp369 & Pro370-Arg685, fused with an N-terminal 9-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: His, Ala39 & Pro370
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit rhBMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315:272.The ED50 for this effect is typically 0.5 - 2 µg/mL in the presence of 75 ng/mL of rhBMP-4.
• Molecular Mass: In SDS-PAGE migrates as 36-39 kDa and 50-55 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 250 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Protein length: 34-685;39-369;370-685 a.a.

Gene Information

• Gene Name: Bmper BMP-binding endothelial regulator [ Mus musculus ]
• Official Symbol: Bmper
• Synonyms: BMPER; BMP-binding endothelial regulator; BMP-binding endothelial regulator protein; mCV2; crossveinless-2; crossveinless 2 homolog; protein crossveinless-2; bone morphogenetic protein-binding endothelial cell precursor-derived regulator; Cv2; CV-2; Crim3; 3110056H04Rik
• Gene ID: 73230
• mRNA Refseq: NM_028472
• Protein Refseq: NP_082748