Active Recombinant Mouse Bgn Protein (Asp38-Lys369), C-6×His tagged

• Cat.No.: Bgn-01M
• Product Overview: Recombinant Mouse Bgn Protein (Asp38-Lys369) with a C-terminal 6-His tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Description: This gene encodes a small, leucine-rich repeat proteoglycan that plays important roles in bone mineralization and connective tissue metabolism. The encoded preproprotein undergoes post-translational processing during which chondroitin sulfate or dermatan sulfate chains are attached before incorporation into the extracellular matrix. Mice lacking the encoded protein exhibit reduced growth rate and acquire diminished bone mass progressively with age.
• Source: CHO
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Bio-activity: Measured by its ability to inhibit the cell growth of mouse 3T3-L1 mouse embryonic fibroblast adipose-like cells. The ED50 for this effect is 0.75-3.5 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 38 kDa
  SDS-PAGE: 45-90 kDa, reducing conditions
• Protein length: Asp38-Lys369
• N-terminal Sequence Analysis: Asp38
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: Bgn biglycan [ Mus musculus (house mouse) ]
• Official Symbol: Bgn
• Synonyms: Bgn; biglycan; BG; PGI; DSPG1; PG-S1; SLRR1A; biglycan; bone/cartilage proteoglycan I
• Gene ID: 12111
• mRNA Refseq: NM_007542
• Protein Refseq: NP_031568
• UniProt ID: P28653