Active Recombinant Mouse Alcam protein(Met1-Lys527), His&hFc-tagged

• Cat.No.: Alcam-624M
• Product Overview: Recombinant Mouse ALCAM (NP_033785.1)(Met 1-Lys 527) precursor was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met1-Lys527
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized mouse CD6-His at 10 μg/ml (100 μl/well) can bind mouse ALCAM-Fc2h, The EC50 of mouse ALCAM-Fc2h is 0.12-0.28 μg/ml.
• Molecular Mass: The recombinant mouse ALCAM/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 748 amino acids and has a predicted molecular mass of 84.2 kDa. Due to glycosylation, in SDS-PAGE under reducing conditions, rmALCAM/Fc migRates with approximately molecular mass of 105-110 kDa & 210 kDa corresponding to the monomer and homodimer.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Alcam activated leukocyte cell adhesion molecule [ Mus musculus ]
• Official Symbol: Alcam
• Synonyms: ALCAM; activated leukocyte cell adhesion molecule; CD166 antigen; BEN; SC1; MuSC; CD166; AI853494; DM-GRASP; MGC27910
• Gene ID: 11658
• mRNA Refseq: NM_009655
• Protein Refseq: NP_033785