Active Recombinant Mouse Adiponectin, C1Q And Collagen Domain Containing, His-tagged

• Cat.No.: Adipoq-5600M
• Product Overview: Recombinant Mouse Adipoqprotein was expressed in Murine myeloma cell line with a C-terminal 6-His tag.Ala111-Asn247 & Tyr112-Asn247.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 111-247;112-247 a.a.
• Description: Adipoq,alternatively named Adipocyte Complement-Related Protein of 30 kDa (Acrp30),shares structural similarity with complement factor C1q and is a member ofthe family of defense collagens. It is secreted exclusively by differentiatedAdipoq and circulates at high concentrations (in μg/mL range). Adipoq has amodular structure comprising an N-terminal collagenous domain with multiplecollagen triple helix repeats, followed by a C-terminal C1q-like globulardomain.
• Form: Supplied as a 0.2 μmfiltered solution in PBS.
• N-terminalSequence: Ala111 & Tyr112
• Molecular Weight: 16.7 kDa
• Activity: Measuredby its ability to induce TIMP-1 secretion by mouse macrophages. Kumada, M. etal. (2004) Circulation 109:2046. The ED50 for this effect istypically 5-20 μg/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 19-23kDa, reducing conditions
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt,-20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Adipoq adiponectin, C1Q andcollagen domain containing [ Mus musculus ]
• Official Symbol: Adipoq
• Synonyms: Adipoq; adiponectin,C1Q and collagen domain containing; APN; Acdc; apM1; 30kDa; GBP28; adipo;Acrp30; adiponectin; adipocyte-specific protein AdipoQ; adipocyte complementrelated protein; 30 kDa adipocyte complement-related protein; adipocytecomplement-related 30 kDa protein; adipocyte, C1Q and collagen domaincontaining; adipocyte, C1q and collagen domain-containing protein
• Gene ID: 11450
• mRNA Refseq: NM_009605
• Protein Refseq: NP_033735
• UniProt ID: Q60994
• Chromosome Location: 16 B3-B4; 16 16.0 cM
• Pathway: Adipocytokinesignaling pathway; Adipogenesis; Leptin and adiponectin; PPAR (Peroxisomeproliferator-activated receptor) signaling pathway; PPAR signaling pathway;Type II diabetes mellitus
• Function: adiponectin-mediatedsignaling pathway; brown fat cell differentiation; cellular response to drug;cellular response to insulin stimulus; detection of oxidative stress; fattyacid beta-oxidation; fatty acid oxidation; glucose homeostasis; glucosemetabolic process; low-density lipoprotein particle clearance; membranedepolarization; membrane hyperpolarization; negative regulation of DNAbiosynthetic process; negative regulation of ERK1 and ERK2 cascade; negativeregulation of I-kappaB kinase/NF-kappaB cascade