Active Recombinant Monkey TNFRSF9 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: TNFRSF9-551RAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Rhesus TNFRSF9 (NP_001253057.1) (Met1-Gln186) was expressed with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Monkey
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Gln186， 401
• Description: CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymph centigradeytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is ass centigradeiated with the tumor necrosis factor receptor–ass centigradeiated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as mon centigradeyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials.
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized rhesus TNFRSF9-Fc at 10 μg/mL (100 μL/well) can bind biotinylated human TNFSF9, the EC50 of biotinylated human TNFSF9 is 20-100 ng/mL.
• Molecular Mass: 44 kDa
• N-terminal Sequence Analysis: Leu 24
• Endotoxin: < 1.0 EU/ μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: Samples are stable for up to twelve months from date of receipt at -70 centigrade. Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: TNFRSF9 TNF receptor superfamily member 9 [ Macaca mulatta (Rhesus monkey) ]
• Official Symbol: TNFRSF9
• Synonyms: TNFRSF9; TNF receptor superfamily member 9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9
• Gene ID: 708281
• mRNA Refseq: NM_001266128
• Protein Refseq: NP_001253057
• UniProt ID: F6W5G6