Active Recombinant Cynomolgus TNFRSF9 protein, hFc-tagged

• Cat.No.: TNFRSF9-191C
• Product Overview: Recombinant Cynomolgus TNFRSF9 protein (XP_005544945.1/NP_001253057.1) (Met1-Gln186) was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Gln186
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA.
  2.Immobilized cynomolgus / rhesus TNFRSF9-Fc at 10μg/mL (100μL/well) can bind biotinylated human TNFSF9, the EC50 of biotinylated human TNFSF9 is 20-100ng/mL.
• Molecular Mass: The recombinant cynomolgus / rhesus TNFRSF9 consists of 401 amino acids and predicts a molecular mass of 44 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: >95% as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.