Active Recombinant Mink ACE2 protein, His-tagged

• Cat.No.: ACE2-2879M
• Product Overview: Recombinant Mink ACE2 protein(Gln 18-Thr 740), fused with His tag, was expressed in HEK293.

Specification

• Species: Mink
• Source: HEK293
• Tag: His
• Protein Length: Gln 18-Thr 740
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM Nacl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Mink ACE2, His Tag immobilized on CM5 Chip can bind SARS-CoV-2 S protein RBD (Y453F), His Tag with an affinity constant of 0.178 μM as determined in a SPR assay (Biacore T200).Mink ACE2, His Tag immobilized on CM5 Chip can bind SARS-CoV-2 S protein RBD, His Tag with an affinity constant of 8.34 μM as determined in a SPR assay (Biacore T200).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 86.3 kDa. The protein migrates as 95-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.