Active Recombinant Influenza A H7N7 (A/Netherlands/219/2003) NA protein

• Cat.No.: NA-0409H
• Product Overview: Recombinant Influenza A H7N7 (A/Netherlands/219/2003) NA protein(Met1-Ser471)(AAR11367.1) was expressed in HEK293.

Specification

• Species: Influenza A H7N7 (A/Netherlands/219/2003)
• Source: HEK293
• Tag: Non
• Protein Length: Met1-Ser471
• Form: Lyophilized from sterile PBS, 187mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, 1%TritonX-100, 6%trehalose, 5.3%manicol, PH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 2’-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid.The specific activity is > 100 U.The specific activity is > 1000 U.One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid per minute at pH 7.5 at 37°C.
• Molecular Mass: The recombinant influenza A H7N7 Neuraminidase comprises 471 amino acids and has a predicted molecular mass of 51.8kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.