Recombinant Influenza A H3N2 (A/Cambodia/e0826360/2020) NA protein(Ile30-Ile469), His-tagged

• Cat.No.: NA-3360V
• Product Overview: Recombinant Influenza A virus (A/Cambodia/e0826360/2020 (H3N2)) neuraminidase (translated amino acids of EPI1837752) (Ile30-Ile469) was expressed in Insect Cells with a vasodilator-stimulated phosphoprotein tetramerization domain at the N-terminus and a polyhistidine tag at the C-terminus.

Specification

• Species: H3N2
• Source: Insect Cells
• Tag: His
• ProteinLength: Ile30-Ile469
• Form: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, 10 % glycerol, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Influenza A virus (A/Cambodia/e0826360/2020 (H3N2)) NA consists of 486 amino acids and predicts a molecular mass of 54.7 kDa. It migrates as an approximately 66.9 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.