Active Recombinant Human WEE1 Protein (Q36-Y432), N-GST-6×His tagged

• Cat.No.: WEE1-15H
• Product Overview: Human WEE1, C-terminal fragment, amino acids Q36-Y432 (as in NCBI/Protein entry NP_001137448.1), N-terminal GST-6×His fusion
  protein with a Thrombin cleavage site, expressed in Sf9 insect cells

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: Q36-Y432
• Description: This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
• Bio-activity: Specific kinase activity (Pi transfer): 2 pmol/μg × min
  ATP-KM: 1.6 μM
• Molecular Mass: 74,902 Da
• Storage: At -80 centigrade
  For complete recovery, mix well and spin before use. Product must not be stored in diluted solutions, aliquots below 10 μL are not advisable. Avoid repeated freeze-thaw cycles!
• Concentration: 0.142 μg/μL (Bradford method using BSA as standard protein)
• Storage Buffer: 50 mM TRIS-HCl pH 8.0, 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20 % glycerol

Gene Information

• Gene Name: WEE1 WEE1 G2 checkpoint kinase [ Homo sapiens (human) ]
• Official Symbol: WEE1
• Synonyms: WEE1; WEE1 G2 checkpoint kinase; WEE1A; WEE1hu; wee1-like protein kinase; WEE1 homolog; WEE1+ homolog; protein kinase; wee1A kinase; EC 2.7.10.2
• Gene ID: 7465
• mRNA Refseq: NM_001143976
• Protein Refseq: NP_001137448
• MIM: 193525
• UniProt ID: P30291