Active Recombinant Human VEGFD protein, His-tagged

• Cat.No.: VEGFD-8113H
• Product Overview: Recombinant Human VEGF-D, His Tag is expressed from human 293 cells (HEK293). It contains AA Phe 93 - Ser 201 (Accession # AAH27948).

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1.Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC).
  The ED50 for this effect is 0.3‑1.6 μg/mL.
  2.Captured VEGFR3/FLT4 Protein, Human on proA Chip can bind VEGF-D with an affinity constant of 39.03 nM as determined in a SPR assay (Biacore T200).
• Molecular Mass: The recombinant mature form of human VEGFD consists of 120 amino acids and has a predicted molecular mass of 13.6 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 20-22 kDa due to glycosylation.
• Protein length: Phe 93-Ser 201
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.